{"id":32,"date":"2022-05-27T15:44:27","date_gmt":"2022-05-27T15:44:27","guid":{"rendered":"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/?page_id=32"},"modified":"2024-01-23T16:08:07","modified_gmt":"2024-01-23T16:08:07","slug":"special-stainings","status":"publish","type":"page","link":"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/","title":{"rendered":"Special Stainings"},"content":{"rendered":"<!-- Preview block -->\n<img loading=\"lazy\" width=\"100%\" height=\"100%\" src=\"\"\/>\n\n<div class=\"block-banner \" data- style=\"background-image: url(https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2022\/07\/22_CP.png)\">\n    <div class=\"inner-container\">\n      <div class=\"text-box\">\n        <h2 class=\"title\">Services<\/h2>\n        <div class=\"text\">\n          <p>Special stainings<\/p>\n\n        <\/div>\n      <\/div>\n    <\/div>\n<\/div>\n\n<style type=\"text\/css\">\n  [data-] {\n    background: ;\n    color: ;\n  }\n<\/style>\n\n\n\n\n<p class=\"has-text-color\" style=\"color:#00c6a5;font-size:25px\"><strong>Special Histochemical Staining<\/strong><\/p>\n\n\n\n<p>Once we have thin, high quality sections mounted on glass slides, we can perform a variety of Special Histochemical staining to highlight specific elements of interest. Our team is highly experienced in the optimization and application of special stains for selective staining of unique tissue elements of interest.<\/p>\n\n\n\n<p>Below is&nbsp;a sampling of the types of&nbsp;staining methods we perform:<\/p>\n\n\n\n<ul><li><strong>Masson Trichrome (MT) staining<\/strong>: This staining method is mainly used to distinguish collagen from muscle tissue, applicable and recommended in fibrosis models, wound healing studies, infarct models and others.<\/li><li><strong>Periodic acid\u2013Schiff (PAS) staining:<\/strong>&nbsp;This staining method is used for staining of polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues.<\/li><li><strong>Toluidine blue staining: <\/strong>This method is used for staining of mast cells that are found in the connective tissue and their cytoplasm contains granules composed of heparin and histamine. Following Toluidine staining, mast cells are stained red-purple and the background is stained blue.<\/li><li><strong>Oil Red O: <\/strong>Oil Red O stain&nbsp;is based on the use of a&nbsp;lysochrome (lipo-soluble dye) and an azo dye used for the visualization of neutral triglycerides and lipids on frozen tissue sections.<\/li><li>And many more!<\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image size-large is-style-default\"><img loading=\"lazy\" width=\"1024\" height=\"330\" src=\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg-1024x330.jpg\" alt=\"\" class=\"wp-image-260\" srcset=\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg-1024x330.jpg 1024w, https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg-300x97.jpg 300w, https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg-768x247.jpg 768w, https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg-1536x495.jpg 1536w, https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg.jpg 1884w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption><em>Human Appendix stained with AFOG, Picrosirius Red and Masson&#8217;s Trichrome (collagen staining)<\/em><\/figcaption><\/figure>\n\n\n\n<p>For custom staining methods, contact our team to discuss and we will accommodate your research needs.&nbsp;<\/p>\n\n\n\n<p style=\"font-size:15px\"><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p class=\"has-light-green-cyan-background-color has-background has-large-font-size\"><strong>Special Staining list available<\/strong> <a href=\"http:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2024\/01\/CPU-SpecialStaining-List.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">here<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Special Histochemical Staining Once we have thin, high quality sections mounted on glass slides, we can perform a variety of Special Histochemical staining to highlight specific elements of interest. Our team is highly experienced in the optimization and application of special stains for selective staining of unique tissue elements of interest. Below is&nbsp;a sampling of &hellip; <a href=\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/\">Continued<\/a><\/p>\n","protected":false},"author":28,"featured_media":0,"parent":24,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"views\/template-900.blade.php","meta":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Special Stainings - Comparative Pathology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Special Stainings - Comparative Pathology\" \/>\n<meta property=\"og:description\" content=\"Special Histochemical Staining Once we have thin, high quality sections mounted on glass slides, we can perform a variety of Special Histochemical staining to highlight specific elements of interest. Our team is highly experienced in the optimization and application of special stains for selective staining of unique tissue elements of interest. Below is&nbsp;a sampling of &hellip; Continued\" \/>\n<meta property=\"og:url\" content=\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/\" \/>\n<meta property=\"og:site_name\" content=\"Comparative Pathology\" \/>\n<meta property=\"article:modified_time\" content=\"2024-01-23T16:08:07+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg-1024x330.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/en\/#website\",\"url\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/en\/\",\"name\":\"Comparative Pathology\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/en\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/#primaryimage\",\"inLanguage\":\"en-US\",\"url\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg.jpg\",\"contentUrl\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/wp-content\/uploads\/sites\/28\/2023\/08\/SSappendixLeg.jpg\",\"width\":1884,\"height\":607},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/#webpage\",\"url\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/\",\"name\":\"Special Stainings - Comparative Pathology\",\"isPartOf\":{\"@id\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/en\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/#primaryimage\"},\"datePublished\":\"2022-05-27T15:44:27+00:00\",\"dateModified\":\"2024-01-23T16:08:07+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Services\",\"item\":\"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Special Stainings\"}]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Special Stainings - Comparative Pathology","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/comparativepathology.imm.medicina.ulisboa.pt\/services\/special-stainings\/","og_locale":"en_US","og_type":"article","og_title":"Special Stainings - Comparative Pathology","og_description":"Special Histochemical Staining Once we have thin, high quality sections mounted on glass slides, we can perform a variety of Special Histochemical staining to highlight specific elements of interest. 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